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Synthesis of structured triglycerides from peanut oil with immobilized lipase
Author(s) -
Soumanou Mohamed M.,
Bornscheuer Uwe T.,
Menge Ulrich,
Schmid Rolf D.
Publication year - 1997
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-997-0101-5
Subject(s) - interesterified fat , triolein , rhizomucor miehei , lipase , peanut oil , chemistry , hydrolysis , triacylglycerol lipase , food science , chromatography , enzyme , biochemistry , organic chemistry , raw material
Structured triglycerides (ST) that contain medium‐ and long‐chain fatty acids were synthesized by lipase‐catalyzed interesterification between tricaprylin and peanut oil. To select appropriate enzymes, we investigated nine commercial lipase preparations for their ability to hydrolyze pure triglycerides as well as natural oils. Three microbial lipases from Rhizomucor miehei (RML), Candida sp. (CSL), and Chromobacterium viscosum (CVL) gave good results, and immobilized preparations were used in the interesterification. RML gave the highest yields of ST (73%, 40°C), although its hydrolytic activity toward triolein was low. As the temperature was raised to 50°C, the yield of ST increased to 79%. After 120 h reaction time, remaining activities were high for CSL (71%), moderate for CVL (48%), and low for RML (20%).