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Esterification patterns of lipases for synthesizing tricaproylglycerols in organic solvent
Author(s) -
Kwon Dae Young,
Song Hyo Nam,
Yoon Suk Hoo
Publication year - 1997
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-997-0059-3
Subject(s) - organic solvent , solvent , chemistry , organic chemistry , environmental science , biochemical engineering , chemical engineering , engineering
To investigate the synthetic patterns of triglyceride (triacylglycerol) by lipases in organic solvent, esterification patterns of triglyceride, diglyceride, and monoglyceride were monitored at various reaction times with 10 lipases. As a model study, tricaprin was synthesized from glycerol and capric acids (C 10:0 ) in isooctane. Lipases that were known to give nonspecific hydrolysis in aqueous solvent, such as lipase from Candida cylindracea , Lipase OF‐360 (from C. rugosa ), and Lipase MY ( C. rugosa ) showed nonspecific synthesis of tricaprin in organic solvent (Group I). There are two groups for esterifying trigly cerides in organic solvent with 1,3‐specific lipases: one consists of the lipases from Rhizomucor miehei, Pseudomonas aeruginosa (Lipase PS), and Chromobacterium viscosum (Lipase CV) (Group II), and another (Group III) is represented by Lipase AP ( Aspergillus niger ), Lipase FAP‐15 ( Rhizopus javanicus ), and Lipase D ( R. delemar ). Although both groups showed 1,3‐specific hydrolysis in aqueous solvent, Group III has stricter 1,3‐specificity for the synthesis of tricaprin from dicaprin.