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Enrichment of γ‐linolenic acid from borage oil via lipase‐catalyzed reactions
Author(s) -
Huang FangCheng,
Ju YiHsu,
Huang ChengWei
Publication year - 1997
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-997-0014-3
Subject(s) - glyceride , chemistry , lipase , hydrolysis , candida rugosa , saponification , fatty acid , organic chemistry , chromatography , catalysis , linolenic acid , enzyme , linoleic acid
Three lipase‐catalyzed reactions were utilized to enrich γ‐linolenic acid in borage oil: (i) selective hydrolysis in isooctane by Candida rugosa lipase immobilized on microporous polypropylene, (ii) selective esterification of free fatty acid from saponified borage oil and n ‐butanol by Lipozyme IM‐20, and (iii) acidolysis of the products of the previous two reactions, that is, unhydrolyzed acylglycerols and unesterified free fatty acid. In the selective hydrolysis, γ‐linolenic acid content could be raised from 23.6 mol% in borage oil to 51.7% in the unhydrolyzed acylglycerols. On the other hand, γ‐linolenic acid content in free fatty acid could be increased to 87% after selective esterification. Products with 65% γ‐linolenic acid in their acylglycerols were obtained by means of the acidolysis reaction.