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Hydrolytic Activity of Castor Bean Powder: Effect of Gum Arabic, Lipase and Oil Concentrations
Author(s) -
Salaberría Florencia,
Palla Camila,
Carrín María Elena
Publication year - 2017
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-017-2976-0
Subject(s) - lipase , gum arabic , sunflower oil , castor oil , hydrolysis , chemistry , arabic , food science , aqueous two phase system , pulmonary surfactant , vegetable oil , aqueous solution , chromatography , enzyme , biochemistry , organic chemistry , linguistics , philosophy
Lipase activity from castor bean seed powders was evaluated in hydrolysis reactions at 37 °C. The effects of different concentrations of lipase powder (LP), substrate (high oleic sunflower oil, O ) and surfactant (gum arabic, A ) on lipase activity ( R ) were assessed using experimental designs. Considered variable bounds were: 0.05–0.15 g LP , 0.07–0.20 oil:aqueous phase (w/w) and 0–0.025 g gum arabic/mL. All variables had significant effects on the transformed response, R 1/2 . The most important result was the negative effect of gum arabic in lipase activity, even when high oil concentrations were used. Experimental lipase activities involved in this work were within 0.32–16.90 mmol FFA /g oil ·g LP ·h. Using 0.05 g LP and 0.20 oil:aqueous phase (w/w) without gum arabic, the activity of 20.47 ± 7.19 mmol FFA /g oil ·g LP ·h was reached.