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Tandem MS Characterization of Endosperm Lipid Profile in Isogenic Waxy Wheat Versus Wildtype Parent Cultivars
Author(s) -
Brewer Lauren R.,
Wetzel David L.
Publication year - 2016
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-016-2823-8
Subject(s) - endosperm , cultivar , glycolipid , food science , biology , chemistry , botany , biochemistry
Among bread quality wheat cultivars, the percentage of lipids is minor, but its potential contribution to desirable traits is significant; particularly the glycolipid fraction. The lipid profile of total lipid extracts from isogenic waxy wheat kernels reveals the genetic expression compared with extracts from the corresponding wildtype wheat kernels. The collateral enhancement of desirable lipid enhancement of waxy wheats that accompanies the genetically defined carbohydrate difference is the subject of the analytical efforts described in this paper. Tandem mass spectrometry directly applied to total extract enables identification and measurement of acyl group populations within each lipid class. Significant increases were observed in glycolipid content for waxy wheats and the N11 waxy (hexaploid) triglycerides were significantly increased vs the wildtype parent.