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13‐Hydroxy‐9 Z ,15 Z ‐Octadecadienoic Acid Production by Recombinant Cells Expressing Lactobacillus acidophilus 13‐Hydratase
Author(s) -
Kang WooRi,
Park ChulSoon,
Shin KyungChul,
Kim KyoungRok,
Oh DeokKun
Publication year - 2016
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-016-2809-6
Subject(s) - recombinant dna , yield (engineering) , escherichia coli , fermentation , lactobacillus acidophilus , biochemistry , chemistry , methanol , bacteria , biology , food science , gene , probiotic , organic chemistry , materials science , genetics , metallurgy
Recombinant Escherichia coli cells expressing linoleate 13‐hydratase from Lactobacillus acidophilus were permeabilized by treating with 0.2 M NaCl. The optimal conditions for the production of 13‐hydroxy‐9,15( Z , Z )‐octadecadienoic acid (13‐HODE) from α ‐linolenic acid by permeabilized recombinant cells were pH 6.0, 40 °C, 7.5 % (v/v) methanol, 60 g/l permeabilized cells, and 15 g/l α ‐linolenic acid. Under these conditions, permeabilized cells produced 7.5 g/l 13‐HODE after 6 h, with a conversion yield of 50 % (w/w) and a volumetric productivity of 1.25 g/l/h. These values were 161 and 160 % of those obtained by nonpermeabilized cells, respectively. To the best of our knowledge, this is the first report on the process optimization for the biotechnological production of 13‐HODE.