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A Comparative Study of Phospholipase A 1 and Phospholipase C on Soybean Oil Degumming
Author(s) -
Jiang Xiaofei,
Chang Ming,
Wang Xiaosan,
Jin Qingzhe,
Wang Xingguo
Publication year - 2014
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-014-2555-6
Subject(s) - phospholipase a1 , soybean oil , phospholipase , hydrolysis , chemistry , phosphatidylcholine , phosphatidylethanolamine , phospholipid , fatty acid , phospholipase c , enzyme , diacylglycerol kinase , chromatography , refining (metallurgy) , biochemistry , phospholipase a2 , phospholipase a , food science , membrane , protein kinase c
The degumming of crude soybean oil with phospholipase A 1 (PLA 1 ) and phospholipase C (PLC) was studied, and optimal conditions were obtained for each enzyme. During degumming with PLA 1 , more fatty acid was found in the oil than would be expected by hydrolysis of only the terminal fatty acid chains, and glycerophosphophorylcholine and glycerophosphoethanolamine were detected in the gums. These observations indicate that acyl‐migration of phospholipid fatty acids occurred during PLA 1 degumming. In addition, results showed that PLA 1 degumming was capable of reducing the phosphorus content in the oil to levels acceptable for physical refining (<10 mg/kg). During degumming with PLC, an increase of 1,2‐diacylglycerol was found, as most phosphatidylcholine and phosphatidylethanolamine were hydrolyzed by this enzyme. Treatment with either enzyme slightly decreased the oxidative stability of the oil and most metals were separated with the gums fraction.