Premium
Trigonella foenum ‐ graecum L. Seed Oil Obtained by Supercritical CO 2 Extraction
Author(s) -
Yang Renming,
Wang Honglun,
Jing Nianhua,
Ding Chenxu,
Suo Yourui,
You Jinmao
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-012-2123-x
Subject(s) - trigonella , extraction (chemistry) , chemistry , supercritical fluid , response surface methodology , antioxidant , supercritical carbon dioxide , chromatography , central composite design , supercritical fluid extraction , yield (engineering) , botany , materials science , biochemistry , organic chemistry , biology , metallurgy
Supercritical CO 2 extraction (SC‐CO 2 ) of fenugreek ( Trigonella foenum ‐ graecum L.) seed oil and its chemical composition and antioxidant activity were investigated. A central composite design combined with response surface methodology was used to study extraction conditions including pressure, temperature, and time. The optimum extraction conditions were 28.5 MPa extraction pressure, 41 °C extraction temperature, and 118 min extraction time, where 3.78 % yield was predicted. Fenugreek seed oil extracted under optimum conditions by SC‐CO 2 was mainly composed of 28.3 % C18:3, 33.45 % C18:2, 9.89 % C16, 8.1 % C18:1, 3.7 % C18, 0.71 % C20, and 0.61 % C22. The fenugreek oil was rich in unsaturated fatty acids (nearly 70 % of the total fatty acids), and polyunsaturated fatty acids accounted for 61.42 % (mass percentage) of the total amount. The 2,2‐diphenyl‐1‐picrylhydrazyl radical‐scavenging activity increased from 12.5 to 88.4 % when the concentration was increased from 1 to 12 mg/ml. The reducing power of the seed oil was concentration‐dependent. The antioxidant activity of the supercritical fluid extraction extract was superior to those obtained by Soxhlet extraction.