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Enzymatic Synthesis of an Isopropyl Ester by Alcoholysis of Camellia Oil
Author(s) -
Liu Liping,
Qin Xiaoli,
Ning Zhengxiang,
Wang Yonghua,
Yang Bo
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-012-2019-9
Subject(s) - chemistry , isopropyl , molar ratio , camellia , substrate (aquarium) , organic chemistry , distillation , catalysis , botany , oceanography , biology , geology
A camellia oil‐based isopropyl ester (CO‐IPE) was successfully synthesized by enzymatic alcoholysis with camellia oil (CO), and its physiochemical properties were analyzed. Three commercial immobilized lipases (Lipozyme RM IM, Lipozyme TL IM and Novozym 435) were screened, and Novozym 435 was the best one. The optimal reaction conditions were achieved at 240 U/g of Novozym 435 loading, a substrate molar ratio of 5:1 (isopropanol/CO), and 24 h of reaction time at 55 °C. Under the above conditions, the content of CO‐IPE was obtained as 89.83%. Purity of CO‐IPE further increased to be 96.95% after separation by rotary evaporation and molecular distillation. The viscosity of the synthesized CO‐IPE showed itself to be about six times lower than that of CO, and the refractive index of the CO‐IPE (1.449) was nearer to 1 in contrast to that of CO. It suggested that CO‐IPE could be more intensively applied in the cosmetic industry.