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Determination of Unsaponifiable Constituents of Deodorizer Distillates by GC–MS
Author(s) -
Naz Saba,
Sherazi S. T. H.,
Talpur Farah N.,
Talpur M. Younis,
Kara Huseyin
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-011-2000-z
Subject(s) - unsaponifiable , squalene , chemistry , distillation , chromatography , saponification , gas chromatography–mass spectrometry , fraction (chemistry) , canola , gas chromatography , mass spectrometry , food science , organic chemistry
Deodorizer distillate is an important by‐product obtained during deodorization in the edible oil industries. It is a complex mixture of many health beneficial constituents like phytosterols, tocopherols and squalene. In the present study a simple gas chromatographic method with mass spectrometry was used for the separation, detection and quantification of different components present in the deodorizer distillate in a very short analysis time of 18 min. A simple saponification procedure without derivatization was used for their analysis followed by GC–MS analysis. Phytosterols concentration (21.27–25.53%) was the most abundant in canola and palm distillate samples whereas, squalene and tocopherol were present in concentration ranges of 2.89–13.21% and 1.29–5.81%, respectively. The present study revealed that the unsaponifiable fraction of deodorizer distillate could be used in cosmetic preparations due to its appreciable amount of bioactive constituents.