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Enzymatic Production of Monoacylglycerols (MAG) and Diacylglycerols (DAG) from Fish Oil in a Solvent‐Free System
Author(s) -
Feltes Maria Manuela Camino,
Villeneuve Pierre,
Baréa Bruno,
Barouh Nathalie,
Oliveira J. Vladimir,
Oliveira Débora,
Ninow Jorge Luiz
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-011-1998-2
Subject(s) - chemistry , glycerol , fish oil , glyceride , monoacylglycerol lipase , lipase , eicosapentaenoic acid , solvent , chromatography , substrate (aquarium) , monoglyceride , organic chemistry , enzyme , polyunsaturated fatty acid , biochemistry , fatty acid , fish <actinopterygii> , fishery , endocannabinoid system , receptor , oceanography , biology , geology
Mono‐ (MAG) and diacylglycerols (DAG) are of nutritional interest. MAG and DAG containing eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were produced in a solvent‐free system via glycerolysis of menhaden oil catalyzed by Novozym 435. The effect of the molar ratio of glycerol to oil, enzyme concentration, and reaction temperature on MAG and DAG production was assessed. The optimal temperature was in the range of 55–70 °C for production of both acylglycerols. The increase in the substrates molar ratio led to a decrease in MAG and DAG content. The enzyme concentration was fixed at the lowest level evaluated (5%, by weight of substrates). High content of MAG (25% by weight) and DAG (41% by weight) containing, respectively, 12.46% EPA and 11.16% DHA, and 14.57% EPA and 13.70% DHA, were produced after 24 h at 70 °C, with 5% of lipase (by weight of substrate) and a glycerol‐to‐oil molar ratio of 1:1. For this reaction, a molar triacylglycerol (TAG) conversion of about 60% was achieved at equilibrium (10 h).