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Utilization of Agro‐industrial Residues for Poly(3‐hydroxyalkanoate) Production by Pseudomonas aeruginosa 42A2 (NCIMB 40045): Optimization of Culture Medium
Author(s) -
RodríguezCarmona E.,
Bastida J.,
Manresa A.
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-011-1897-6
Subject(s) - polyhydroxyalkanoates , biomass (ecology) , pseudomonas aeruginosa , productivity , central composite design , composition (language) , food science , chemistry , carbon source , pseudomonas , response surface methodology , pulp and paper industry , carbon fibers , biochemistry , chromatography , biology , composite number , bacteria , materials science , agronomy , engineering , composite material , art , genetics , literature , economics , macroeconomics
In this study, central composite design and a response surface methodology are shown to be useful tools for medium optimization, in cultures of Pseudomonas aeruginosa 42A2 using an industrial oil byproduct, for polyhydroxyalkanoate (PHA) production. The optimum medium composition for PHA production includes: 80 g/L of the carbon source; 18.2 g/L of NaNO 3 and 3.3 g/L of K 2 HPO 4 /1.6 g/L KH 2 PO 4 ( R 2 = 0.989). The models were validated experimentally by cultivating Pseudomonas aeruginosa 42A2 in optimum media that yielded similar biomass (18.73 g/L) and PHA (4.63 g/L) concentrations to the predicted values. The optimized media showed an increase in biomass productivity from 0.06 to 0.39 g biomass/L h, and an increase in PHA productivity from 0.03 to 0.1 g PHA/L h.