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Preparation of Diacylglycerol‐Enriched Oil from Free Fatty Acids Using Lecitase Ultra‐Catalyzed Esterification
Author(s) -
Wang Lili,
Wang Yong,
Hu Changying,
Cao Qian,
Yang Xiaohui,
Zhao Mouming
Publication year - 2011
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-011-1821-0
Subject(s) - diacylglycerol kinase , chemistry , glyceride , chromatography , glycerol , catalysis , yield (engineering) , molar ratio , diglyceride , fatty acid , organic chemistry , enzyme , materials science , protein kinase c , metallurgy
Production of diacylglycerol‐enriched oil by esterification of free fatty acids (FFA) with glycerol (GLY) using phospholipase A1 (Lecitase Ultra) was investigated in this work. The variables including reaction time (2–10 h), water content (2–14 wt%, FFA and GLY mass), enzyme load (10–120 U/g, FFA and GLY mass), reaction temperature (30–70 °C) and mole ratio of GLY to FFA (0.5–2.5) were studied. The optimum conditions obtained were as follows: reaction temperature 40 °C, water content 8 wt%, reaction time 6 h, molar ratio of GLY to FFA 2.0, and an enzyme load of 80 U/g. Under these conditions, the esterification efficiency (EE) of free fatty acids was 74.8%. The compositions of the FFA and acylglycerols of the upper oil layer (crude diacylglycerol) of the reaction mixture were determined using a high temperature gas chromatograph (GC). The crude diacylglycerol from the selected conditions was molecularly distilled at 170 °C evaporator temperatures to produce a diacylglycerol‐enrich oil (DEO) with a purity of 83.1% and a yield of 42.7%.