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Production of MLM‐Type Structured Lipids Catalyzed by Immobilized Heterologous Rhizopus oryzae Lipase
Author(s) -
Nunes P. A.,
PiresCabral P.,
Guillén M.,
Valero F.,
Luna D.,
FerreiraDias S.
Publication year - 2011
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-010-1702-y
Subject(s) - rhizopus oryzae , lipase , capric acid , chemistry , pichia pastoris , fatty acid , hexane , caprylic acid , chromatography , food science , fermentation , biochemistry , enzyme , lauric acid , recombinant dna , gene
This work aims to produce triacylglycerols (TAG) containing a medium‐chain fatty acid (M) at positions sn ‐1,3 and a long‐chain fatty acid (L) at sn ‐2 position, i.e. TAG of MLM type, by acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, catalyzed by 1,3‐selective Rhizopus oryzae heterologous lipase (rROL) immobilized in Eupergit ® C and modified sepiolite. This lipase was produced by the methylotrophic yeast Pichia pastoris . Reactions were performed at 25 and 40 °C, for 24 h, either in solvent‐free or in n ‐hexane media, at a molar ratio 1:2 (olive oil:free fatty acid). Higher incorporations of C8:0 (21.6 mol%) and C10:0 (34.8 mol%) into the TAG were attained in solvent‐free media, at 40 °C, when rROL immobilized in Eupergit ® C was used. In organic media, at 40 °C, only 15.9 and 14.1 mol%, incorporation of C8:0 or C10:0 were, respectively observed. Lower incorporations were attained for both acids (3.4–7.0 mol%) when native ROL (nROL) in both supports and rROL in modified sepiolite were used. rROL in Eupergit ® C maintained its activity during the first four or three 23‐h batches, respectively when C8:0 (half‐life time, t 1/2 = 159 h) or C10:0 ( t 1/2 = 136 h) were used, decreasing thereafter following a time delay model.