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Triarachidonoyl Glycerol Purification Process
Author(s) -
VenegasVenegas E.,
RincónCervera M. A.,
GuilGuerrero J. L.
Publication year - 2011
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-010-1680-0
Subject(s) - chromatography , fraction (chemistry) , hydrolysis , gravimetric analysis , chemistry , arachidonic acid , elution , gradient elution , glycerol , process (computing) , high performance liquid chromatography , biochemistry , enzyme , organic chemistry , computer science , operating system
Purification of arachidonic acid (AA, 20:4n‐6) from different sources has been previously reported, but in most cases AA is obtained after triacylglycerol (TAG) hydrolysis. In this work, gravimetric normal‐phase chromatography with gradient elution has been used to purify an AA‐enriched fraction of TAG from the commercial single cell oil named ARASCO (38–44% AA content). A TAG fraction with more than 90% AA content was obtained, employing appropriate solvents for alimentary processing. The process was scaled up with satisfactory results. Due to the use of food‐safe solvents in the whole process, it could be applied with alimentary or pharmaceutical purposes.