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Regiospecific Analysis of Mono‐ and Diglycerides in Glycerolysis Products by GC × GC‐TOF‐MS
Author(s) -
Indrasti Dias,
Che Man Yaakob B.,
Chin Sung Tong,
Mustafa Shuhaimi,
Mat Hashim Dzulkifly,
Abdul Manaf Marina
Publication year - 2010
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-010-1614-x
Subject(s) - glycerol , chemistry , chromatography , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , essential oil , organic chemistry
Comprehensive bidimensional gas chromatography coupled with time‐of‐flight mass spectrometry (GC × GC‐TOF‐MS) was used for the characterization of regiospecific mono‐ and diglycerides (MG‐DG) content in the glycerolysis products derived from five different lipids included lard (LA), sun flower seed oil (SF), corn oil (CO), butter (BU), and palm oil (PA). The combination of fast and high temperature non‐orthogonal column set namely DB17ht (6 m × 0.10 mm × 0.10 μm) as the primary column and SLB‐5 ms (60 cm × 0.10 mm × 0.10 μm) as the secondary column was applied in this work. System configuration involved high oven ramp temperature to obtain precise mass spectral identification and highest effluent's resolution. 3‐Monopalmitoyl‐ sn ‐glycerol (MG 3‐C16) was the highest concentration in LA, BU and PA while monostearoyl‐ sn ‐glycerol (MG C18) in CO and 1,3‐dilinoleol‐ rac ‐glycerol (DG C18:2c) in SF. Principal component analysis accounted 82% of variance using combination of PC1 and PC2. The presence of monostearoyl‐ sn ‐glycerol (MG C18), 3‐Monopalmitoyl‐ sn ‐glycerol (MG 3‐C16), 1,3‐dilinoleol‐ rac ‐glycerol (DG C18:2c), 1,3‐dipalmitoyl‐glycerol (DG 1,3‐C16), and 1,3‐dielaidin (DG C18:1t) caused differentiation of the samples tested.