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Optimization of the Conditions for Removing Cholesterol from Cod Liver Oil by β‐Cyclodextrin Crosslinked with Adipic Acid
Author(s) -
Chang Yoon Hyuk,
Lee Jung Eun,
Kwak HaeSoo
Publication year - 2010
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-010-1555-4
Subject(s) - distilled water , mixing (physics) , adipic acid , chemistry , cyclodextrin , cholesterol , chromatography , soybean oil , food science , biochemistry , organic chemistry , physics , quantum mechanics
This study was conducted to find the optimum conditions for β‐cyclodextrin (β‐CD) crosslinked by adipic acid to remove cholesterol from cod liver oil. The cholesterol content of the non‐treated cod liver oil was 554.51 mg/100 g oil. The different factors considered were concentrations of crosslinked β‐CD, mixing temperature, ratio of cod liver oil to distilled water, mixing time, and mixing speed. The optimum conditions for cholesterol removal from cod liver oil using crosslinked β‐CD were a 1:2 ratio of cod liver oil to distilled water, 25% (crosslinked β‐CD/distilled water, w/v) crosslinked β‐CD concentration, 20 min mixing time, 400 rpm mixing speed and 60 °C mixing temperature with about 87% cholesterol removal. In a recycling study, cholesterol removal from the cod liver oil with recycled crosslinked β‐CD in the first recycling trial was 85.09%, which was slightly lower than that with new crosslinked β‐CD (87.27%). Up to three time trials, more than 82% cholesterol removal was observed.