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The Plackett–Burman Design for Evaluating the Production of Polyunsaturated Fatty Acids by Physcomitrella patens
Author(s) -
Chodok Pichit,
KanjanaOpas Akkharawit,
Kaewsuwan Songsri
Publication year - 2010
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1532-y
Subject(s) - polyunsaturated fatty acid , physcomitrella patens , food science , arachidonic acid , eicosapentaenoic acid , chemistry , sucrose , plackett–burman design , linolenic acid , linoleic acid , fatty acid , biochemistry , fermentation , mutant , gene , enzyme
Identification of the parameters that had significant effects on polyunsaturated fatty acids (PUFAs) and biomass production by the moss Physcomitrella patens was performed using nine culture variables (temperature, agitation speed, pH, sucrose, di‐ammonium tartrate, CaCl 2 ·2H 2 O, MgSO 4 ·7H 2 O, KH 2 PO 4 and KNO 3 ) with the statistical design technique of Plackett–Burman. Statistical analysis revealed that two physical variables (pH and temperature) had significant effects on the production of both biomass and PUFAs (linoleic acid, LA; γ‐linolenic acid, GLA; α‐linolenic acid, ALA; eicosadienoic acid, EDA; di‐homo‐γ‐linolenic acid, DHGLA; arachidonic acid, ARA; eicosapentaenoic acid, EPA). Three nutritional variables (sucrose, CaCl 2 and MgSO 4 ) had an influence only on the production of some of the PUFAs. Of the two levels used in this study, higher concentrations of sucrose had a positive effect on LA, ARA and EPA production, whereas higher concentrations of metal ions (CaCl 2 and MgSO 4 ) had a negative effect only on ARA and EPA production. After adjustment by multiple linear regression, it can be concluded that pH, temperature, sucrose, CaCl 2 and MgSO 4 were the most statistically significant parameters for the growth of P. patens and for PUFA production by this moss.