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Scalable Preparation of High Purity Rutin Fatty Acid Esters
Author(s) -
Lue BenaMarie,
Guo Zheng,
Glasius Marianne,
Xu Xuebing
Publication year - 2010
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1471-7
Subject(s) - chemistry , rutin , lauric acid , acetone , extraction (chemistry) , chromatography , fatty acid , bioconversion , acylation , palmitic acid , organic chemistry , ethyl acetate , substrate (aquarium) , solvent , fermentation , catalysis , oceanography , antioxidant , geology
Investigations into expanded uses of modified flavonoids are often limited by the availability of these high purity compounds. As such, a simple, effective and relatively fast method for isolation of gram quantities of both long and medium chain fatty acid esters of rutin following scaled‐up biosynthesis reactions was established. Acylation reactions of rutin and palmitic or lauric acids were efficient in systems containing dried acetone and molecular sieves, yielding from 70–77% bioconversion after 96 h. Thereafter, high purity isolates (>97%) were easily obtained in significant quantities following a two‐step solvent purification procedure whereby excess fatty acid substrate was first removed in a heptane/water (4:1, v/v) system, followed by selective ester extraction using an ethyl acetate/water system (1:6, v/v) at elevated temperature.