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Further Lipid Profiling of the Oil from the Mophane Caterpillar, Imbrasia belina
Author(s) -
Yeboah S. O.,
Mitei Y. C.
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1443-y
Subject(s) - unsaponifiable , campesterol , stigmasterol , chemistry , chromatography , food science , cholesterol , sterol , biochemistry
A comprehensive lipid profiling of the oil from the edible mophane caterpillar, Imbrasia belina , has been carried out as part of the study of the nutritional value of the caterpillar. GC‐MS analysis revealed the composition of the major FA classes as 18:3 (29.98%), 16:0 (25.64%), 18:1 (17.97%), 18:0 (12.49%) and 18:2 (11.81%), which was in agreement with reported GC‐FID analysis of the phane oil. ESI‐FTICR mass spectrometric analysis showed phane oil to contain 20 TAG classes, with C54:4 (14.59%), C52:3 (14.71%) and C52:2 (10.49%) being the dominant classes, whilst 13 C‐NMR studies of the TAGs regiochemistry showed that occupancy of the sn ‐2 position was dominated by linolenyl and linoleoyl groups whereas the sn‐ 1/3 positions were dominated by saturated groups. Normal‐phase HPLC analysis of the unsaponifiable matter showed the presence of α ‐tocopherol (71.39 μg/g) and γ ‐tocopherol (1.66 μg/g) as the only tocol content in phane oil. GC‐MS analysis of the total acetylated unsaponifiable matter gave the relative composition of the major sterols as cholesterol (53.77%), β ‐sitosterol (24.16%), 22‐dehydrocholesterol (14.58%) and campesterol (6.26%), whilst GC‐MS analysis of an SPE pre‐fractionated unsaponifiable matter gave the absolute 4‐desmethylsterol content (μg/g) as cholesterol (4482.44), β ‐sitosterol (1861.95), 22‐dehydrocholesterol (1274.53), campesterol (503.83) and stigmasterol (21.78). Perhaps the adverse effect of such high dietary cholesterol content on humans could be mitigated by the presence of the substantial amounts of β ‐sitosterol and campesterol which are known to be blood plasma cholesterol lowering phytosterols.