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Quantitative Analysis of Dairy Phospholipids by 31 P NMR
Author(s) -
MacKenzie Andrew,
Vyssotski Mikhail,
Nekrasov Eduard
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1403-6
Subject(s) - phosphatidylethanolamine , phospholipid , phosphatidylcholine , sphingomyelin , chromatography , chemistry , phosphatidylserine , quantitative analysis (chemistry) , lecithin , proton nmr , analytical chemistry (journal) , cholesterol , biochemistry , membrane , stereochemistry
31 P NMR analysis of samples prepared in a sodium cholate detergent system was assessed as a method for the quantitative measurement of dairy phospholipids. Major phospholipid (PL) classes measured included: phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SM) and dihydrosphingomyelin (DHSM). The 31 P NMR method was validated by comparison with a quantitative two‐dimensional thin‐layer chromatography (2D‐TLC) technique. The 2D‐TLC system was more sensitive, able to detect some minor compounds not observed by 31 P NMR. However, 31 P NMR is more suited to routine analysis, with sample analysis taking 36 min. The method was also more versatile and sample analysis was possible on high phospholipid containing materials without prior lipid extraction (e.g. buttermilk protein concentrate, beta serum liquid).