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Study of Some Experimental Parameters in the Synthesis of Triacylglycerols with CLA Isomers and Structural Analysis
Author(s) -
Blasi Francesca,
Maurelli Silvia,
Cossignani Lina,
D’Arco Gilda,
Simonetti Maria Stella,
Damiani Pietro
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1390-7
Subject(s) - rhizomucor miehei , conjugated linoleic acid , chemistry , lipase , diacylglycerol kinase , substrate (aquarium) , enzyme , factorial experiment , chromatography , organic chemistry , triacylglycerol lipase , linoleic acid , fatty acid , mathematics , biology , ecology , statistics , protein kinase c
The present research deals with the synthesis of structured triacylglycerols (TAG) by enzymatic treatment of sn ‐1,3‐diacylglycerol ( sn ‐1,3‐DAG) with conjugated linoleic acid (CLA) isomers using the immobilized lipase from Rhizomucor miehei (Lipozyme ® IM) under different experimental conditions. In particular, the influence of reaction parameters, such as temperature, enzymatic load, reaction time and DAG/CLA ratio has been evaluated using an experimental design software with a screening objective. Two responses have been selected, they are the percentage of CLA isomers in total TAG and in the sn ‐2‐ position and a three‐level‐4‐factor fractional factorial experimental design was used to screen the variables. The results showed that the selected experimental variables have an influence on the enzymatic reaction, in particular, the DAG/CLA substrate ratio and the temperature, both of which inversely correlated with CLA incorporation, but also the enzymatic load and the reaction time, both directly correlated with CLA incorporation. The best results for CLA isomer % content both in total TAG (46.3%) and in the sn ‐2‐ position (52.2%) were obtained at 40 °C for 96 h, with 20% enzymatic load and a 0.5 reactive ratio.