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Designs of Bioreactor Systems for Solvent‐Free Lipase‐Catalyzed Synthesis of Fructose–Oleic Acid Esters
Author(s) -
Pyo SangHyun,
Hayes Douglas G.
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1381-8
Subject(s) - bioreactor , oleic acid , chemistry , lipase , fructose , chromatography , batch reactor , catalysis , organic chemistry , nuclear chemistry , biochemistry , enzyme
Fructose–oleic acid esters, biodegradable, biocompatible and biobased surfactants and value‐added products were synthesized under solvent‐free conditions at 65 °C in stirred‐batch mode and using several different bioreactor systems. For a stirred‐tank bioreactor (STBR) using fed‐batch fructose addition and 5.0 wt.% immobilized Rhizomucor miehei lipase (Lipozyme ® IM, Novozymes, Franklinton, NC), the conversion yield was over 80%, and the initial rate of the reaction was comparable to previously obtained results using tert ‐butanol during the initial phase. The bioreactor systems contained a packed “desorption” column (DC) containing fructose crystals and silica gel for delivery of saccharide, and either a STBR or packed‐bed bioreactor (PBBR). The liquid stream, initially containing oleic acid and a mixture of fructose–oleic acid esters at a ratio of 75/25 w/w, was continuously recirculated throughout the system. The PBBR system yielded the highest conversion (84.4%) and rate of reaction subsequent to the addition of 10 wt.% molecular sieves during the latter stage of reaction; however, the reaction rate was several‐fold lower than the batch mode reactions due to the lower fructose concentrations provided by the DC.