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Purification of GLA‐Triglycerides from Evening Primrose Oil by Gravimetric Column Chromatography
Author(s) -
RincónCervera M. A.,
RodríguezGarcía I.,
GuilGuerrero J. L.
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1378-3
Subject(s) - evening primrose oil , chromatography , chemistry , degree of unsaturation , gamma linolenic acid , linolenic acid , hydrolysis , evening primrose , elution , column chromatography , fatty acid , biochemistry , linoleic acid , food science , polyunsaturated fatty acid
Gravimetric normal‐phase silver ion–silica gel column chromatography has been used for the novel application of purification of GLA‐containing triglycerides (GLA‐TGs) from evening primrose seed oil (EPO). Gradient elution with increasing polarity enabled separation of valuable TG species containing γ ‐linolenic acid (GLA, 18:3n‐6). Enzymatic hydrolysis revealed the distribution of fatty acids (FAs) in the isolated TG species, with GLA in the sn ‐2 position in different percentages, depending on the degree of unsaturation. A novelty of this work was the successful use of the procedure to improve the purification of raw GLA species from EPO up to preparative scale, thus enabling use of this methodology for industrial purposes.