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A Rapid, Micro FAME Preparation Method for Vegetable Oil Fatty Acid Analysis by Gas Chromatography
Author(s) -
Lall Rahul K.,
Proctor Andrew,
Jain Vishal P.
Publication year - 2009
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-009-1359-6
Subject(s) - serial dilution , flame ionization detector , gas chromatography , chromatography , vegetable oil , chemistry , fatty acid , soybean oil , fatty acid methyl ester , solvent , sample preparation , hexane , limiting , organic chemistry , food science , catalysis , biodiesel , medicine , alternative medicine , pathology , mechanical engineering , engineering
A 30‐min, micro‐base‐catalyzed method for vegetable oil fatty acid methyl ester (FAME) preparation was developed using only 1 mg of oil sample by limiting the solvent volumes used. This method was primarily developed to quickly analyze fatty acid composition of CLA‐rich soy oil but can be further applicable to pure vegetable oils. Existing base‐catalyzed FAME preparation methods are not appropriate to use because they are either rapid but not micro, or micro but not rapid, or are rapid and micro but use acidification in the final step of FAME preparation, which would isomerize oils containing conjugated fatty acids. Serial dilutions of a mixed commercial FAME reference standard were prepared and analyzed by GC with a flame ionization detector (FID) with maximum instrument sensitivity. The novel method was also used to prepare soy oil FAMEs for GC‐FID analysis. There were no statistically significant differences ( P < 0.05) in fatty acid data from the FAME reference standard dilutions. Similarly, there was no statistical significant difference ( P < 0.05) between results obtained for all the soy oil dilutions and the control method. This technique is a rapid method for preparing small pure oil samples as FAMEs for GC‐FID analysis.