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Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
Author(s) -
Kobayashi Takashi,
Matsuo Takemasa,
Kimura Yukitaka,
Adachi Shuji
Publication year - 2008
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-008-1285-z
Subject(s) - candida antarctica , lipase , chemistry , glycerol , chromatography , thermal stability , immobilized enzyme , enzyme , organic chemistry
The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as a function of time. The glycerol‐containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.