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Purification of arachidonic acid from fungal single‐cell oil via Al 2 O 3 ‐supported CuSO 4 column chromatography
Author(s) -
Zhang YunXiao,
Tao JingChao,
Wang Le
Publication year - 2006
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-006-1254-3
Subject(s) - saponification , column chromatography , chromatography , chemistry , yield (engineering) , urea , acetone , arachidonic acid , fraction (chemistry) , hexane , organic chemistry , materials science , metallurgy , enzyme
A new method has been proven successful to obtain high‐purity arachidonic acid (AA), an important human nutrient, from fungal single‐cell oil via Al 2 O 3 ‐supported CuSO 4 column chromatography; the stationary phase is stable and readily reusable. In the first step, the mixed FA extracted from saponified fungal single‐cell oil, containing 47.3% AA, were purified via urea inclusion to afford a fraction of PUFA containing 74.9% AA with an 85.9% yield. The enriched AA fraction was subsequently passed through an Al 2 O 3 ‐supported CuSO 4 column with a hexane/acetone eluent to provide AA with 90.8% purity at a 46.5% yield. The total yield for the two‐step process was 39.9%.