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Tocopherol distribution and molecular species of triacylglycerols in soybean embryonic axes
Author(s) -
Yoshida Hiromi,
Tomiyama Yuka,
Kanrei Saki,
Mizushina Yoshiyuki
Publication year - 2006
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-006-1211-1
Subject(s) - tocopherol , degree of unsaturation , soy milk , chemistry , high performance liquid chromatography , chromatography , germ , soybean oil , biology , biochemistry , food science , vitamin e , antioxidant , microbiology and biotechnology
Soybean embryonic axes were separated from other tissues, i.e., the cotyledons and seed coat. The molecular species and FA distribution of TAG isolated from total lipids in the embryonic axes were analyzed by a combination of argentation‐TLC and GC, and were investigated in relation to their tocopherol distribution, which was determined by HPLC. The dominant components were γ‐tocopherols, with much smaller amounts of α‐, β‐, and δ‐tocopherols. A modified argentation‐TLC procedure, developed to optimize the separation of the complex mixture of total TAG, provided 16 different groups of TAG, based on both the degree of unsaturation and the total acyl‐chain length of FA groups. With a few exceptions, the major TAG components were S 2 D (6.8–10.3%), SMD (6.9–11.2%), SD 2 (7.2–9.8%), SMT (3.2–7.4%), SDT (11.5–19.5%), D3 (3.5–8.3%), MDT (4.5–7.7%), D 2 T (11.1–20.6%), and DT 2 (8.2–15.7%) (where S denotes saturated FA, M denotes monoenes, D denotes dienes, and T denotes trienes). These results indicate that there were significant differences ( P <0.05) not only in tocopherol distribution but also in the molecular species of TAG among the four cultivars. Therefore, these tissues should be made available as raw materials for soybean‐germ oil or soy milk, based on the differences in the distributions of tocopherol homologs and the molecular species of TAG within the embryonic axes.