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A novel method for solvent fractionation of two CLA isomers
Author(s) -
Uehara Hidetaka,
Suganuma Tomomi,
Negishi Satoshi,
Ueno Satoru,
Sato Kiotaka
Publication year - 2006
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-006-1202-2
Subject(s) - decanoic acid , crystallization , chemistry , solvent , conjugated linoleic acid , acetone , fatty acid , chromatography , linoleic acid , cis–trans isomerism , organic chemistry
Conjugated linoleic acid (CLA) is commercially available as a mixture consisting of almost equal amounts of the cis ‐9,trans‐11‐CLA ( c9 , t 11) and trans ‐10, cis ‐12‐CLA ( t 10, c 12) isomers. Separation of the two isomers is highly significant since each exhibits different biochemical properties. Highly efficient separation could be accomplished by crystallization in acetone (solvent) of the two CLA isomers (solutes) in the presence of medium‐chain fatty‐acid (MCFA) additives. The relative concentration ratios of the two CLA isomers in the solvent‐crystallized materials varied depending on which MCFA were added. Addition of lauric and decanoic acids resulted in the crystals predominantly containing t 10, c 12, whereas octanoic acid yielded those predominantly containing c 9, t 11. We have confirmed that onetime solvent crystallization using decanoic acid and octanoic acid additives increased the t 10, c 12 and c 9, t 11 concentrations, and that repeated solvent crystallization resulted in the ratio of c 9, t 11 to t 10, c 12 of at least 4∶96 or 98∶2.