Premium
Separation of Triacylglycerol Species from Interesterified Oils by High‐Performance Liquid Chromatography
Author(s) -
Lee Jeung Hee,
Jones Kerby C.,
Foglia Thomas A.,
Nuñez Alberto,
Lee Jong Ho,
Kim Yu Mi,
Vu PhuongLan,
Lee KiTeak
Publication year - 2007
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-006-1029-x
Subject(s) - tributyrin , chemistry , chromatography , high performance liquid chromatography , interesterified fat , lipase , soybean oil , organic chemistry , biochemistry , enzyme
Using a 1,3‐regioselective lipase as a catalyst, soybean oil and olive oil were interesterified with the short‐chain triacylglycerol tributyrin (1,2,3‐tributyrylglycerol) to produce mixtures of structured triacylglycerols (SL‐TAG). The SL‐TAG were purified by column chromatography and analyzed by both normal‐phase (silica column; NP SIL ) and reversed‐phase [octadecyl silane (ODS) column] high‐performance liquid chromatography (HPLC). Individual SL‐TAG molecular species were detected by evaporative light‐scattering detection, and characterized by mass spectrometry. NP SIL HPLC successfully separated the newly synthesized SL‐TAG into two groups of TAG: one composed of one butyryl group and two long‐chain fatty acyl groups (from soybean or olive oil); the second was composed of two butyryl groups and one long‐chain fatty acyl group. The SL‐TAG species were further analyzed by reversed‐phase HPLC which gave a more detailed separation of the TAG species present in the two SL‐TAG.