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Monooxygenase system of Bacillus megaterium ALA2: Studies on palmitic acid oxidation products
Author(s) -
Hou Ching T.
Publication year - 2005
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-005-1152-8
Subject(s) - palmitic acid , bacillus megaterium , linoleic acid , bacillus subtilis , strain (injury) , monooxygenase , chemistry , stereochemistry , bacillaceae , biochemistry , bacteria , fatty acid , enzyme , biology , anatomy , cytochrome p450 , genetics
We identified many novel oxygenated FA produced from linoleic acid by microbial strain ALA2: 12,13,17‐trihydroxy‐9(Z)‐octadecenoic acid (12,13,17‐THOA); 12,13,16‐trihydroxy‐9(Z)‐octadecenoic acid (12,13,16‐THOA); 12‐hydroxy‐13,16‐epoxy‐9(Z)‐octadecenoic acid; and 12,17;13,17‐diepoxy‐16‐hydroxy‐9(Z)‐octadecenoic acid. 12,13,17‐THOA, the main product, inhibits the growth of some plant pathogenic fungi. Recently, we reclassified strain ALA2 as Bacillus megaterium ALA2 NRRL B‐21660 and opened a possible link with the well‐studied catalytically self‐sufficient P450 monooxygenase of Bacillus megaterium ATCC 14581 (NRRL B‐3712) and B. subtilis strain 168 (NRRL B‐4219). Now we have found that strain ALA2 also oxidizes palmitic acid into three oxygenated products: 13‐, 14‐, and 15‐hydroxy palmitic acids. This indicates that strain ALA2 also possesses a monooxygenase system similar to the abovementioned well‐known strains. These data facilitate studies on the oxygenase system of strain ALA2.