Modeling of kinetics for the enzymatic hydrolysis of sunflower oil in a high‐pressure reactor | Zendy

Primožič Mateja | Zendy; Habulin Maja | Zendy; Knez Željko | Zendy

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Modeling of kinetics for the enzymatic hydrolysis of sunflower oil in a high‐pressure reactor

Author(s) -

Primožič Mateja,

Habulin Maja,

Knez Željko

Publication year - 2005

Publication title -

journal of the american oil chemists' society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.512

H-Index - 117

eISSN - 1558-9331

pISSN - 0003-021X

DOI - 10.1007/s11746-005-1107-0

Subject(s) - hydrolysis , supercritical fluid , supercritical carbon dioxide , sunflower , lipase , sunflower oil , chemistry , kinetics , enzymatic hydrolysis , solvent , organic chemistry , catalysis , chromatography , chemical engineering , enzyme , biochemistry , mathematics , engineering , physics , combinatorics , quantum mechanics

This study presents a mathematical model for characterizing the Michaelis‐Menten type of sunflower oil hydrolysis, catalyzed by the lipase preparation Lipolase 100T (Novozymes Ays, Bagsvaerd, Denmark). Supercritical carbon dioxide was used as a solvent for this reaction. Computer modeling of the kinetics was done for the enzymatic hydrolysis of sunflower oil in a high‐pressure reactor using the MicroMath Scientist ® program (Micro‐Math Research, St. Louis, MO). On the basis of the experimental results, we presumed the reaction was reversible. The suitability of the model was confirmed statistically with Student's t ‐test. Good agreement was found between the experimental and calculated values for the concentrations of oleic and linoleic acids.

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