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Incorporation of laurate and hydroxylaurate into phosphatidylcholines and acylglycerols in castor microsomes
Author(s) -
Lin JiannTsyh,
Wani Seiji,
He Xiaohua,
Nguyen Tasha,
McKeon Thomas A.
Publication year - 2005
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-005-1099-9
Subject(s) - linolenate , castor oil , glyceride , chemistry , microsome , chromatography , stearate , ricinus , fatty acid , biochemistry , organic chemistry , enzyme
Because castor produces oil with a high content of hydroxyl FA (90% ricinoleate), we were interested in determining the flexibility of castor seed microsomes in incorporating other hydroxyl FA into castor oil. To this end, we incubated the [ 14 C]‐labeled 12:0 FA laurate (La), 11‐hydroxylaurate, and 12‐hydroxylaurate with castor microsomes that were capable of synthesizing castor oil. The molecular species of PC and acylglycerols (AG) incorporating these nonendogenous FA of castor were identified by reversed‐phase C 8 and C 18 HPLC, respectively. [ 14 C]Laurate was incorporated into the molecular species of PC and AG at levels of 10 and 4%, respectively, that of [ 14 C]ricinoleate. Similar to those from the incorporation of six [ 14 C]FA reported previously [ricinoleate (R), oleate (O), linoleate (L), linolenate (Ln), stearate (S), and palmitate (P)], the molecular species of PC incorporating [ 14 C]laureate were LLa‐PC>PLa‐PC>OLa‐PC>LnLa‐PC>SLa‐PC>RLa‐PC. The molecular species of AG incorporating [ 14 C]laurate were RRLa>LaLa>RLa>RLLa>ROLa>LOLa>LLLa>LLa>LLnLa>RSLa>OOLa. The retention times for lipids incorporating laurate were similar to those of lipids incorporating linolenate, because the equivalent carbon numbers of laurate and linolenate are the same. Relative retention times of the molecular species of PC and AG containing laurate are also reported here. The incorporation of 11‐hydroxylaurate and 12‐hydroxylaurate into PC and AG was not detected.