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Enzymatic incorporation of stearic acid into a blend of palm olein and palm kernel oil: Optimization by response surface methodology
Author(s) -
Lumor Stephen E.,
Akoh Casimir C.
Publication year - 2005
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-005-1087-0
Subject(s) - palm kernel oil , stearic acid , substrate (aquarium) , quadratic model , response surface methodology , palm kernel , palm stearin , chemistry , palm oil , hexane , chromatography , molar ratio , mathematics , materials science , organic chemistry , food science , catalysis , oceanography , geology
Response surface methodology was used to model the incorporation of stearic acid into a blend of palm olein and palm kernel oil in hexane using the sn ‐1,3‐regiospecific lipase Lipozyme RM IM. The factors investigated were incubation time, temperature, and substrate molar ratio. A second‐order model with interaction was used to fit the experimental data. The coefficients of determination, R 2 and Q 2 , were 0.96 and 0.90, respectively. The adjusted R 2 was 0.95. The regression probability was less than 0.001, and the model showed no lack of fit. Also, a linear relationship was observed between the predicted and observed values. All parameters studied had positive effects on incorporation of stearic acid, with substrate molar ratio having the greatest effect. The interaction terms of substrate molar ratio with temperature and time also had positive effects on incorporation, whereas the effect of the squared term of substrate molar ratio was negative. The quadratic terms of temperature and time, as well as their interaction term, had no significant effect on incorporation at α 0.05 . Model verification was done by performing a chi‐square test, which showed that there was no significant difference between predicted values and a new set of observed responses.