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Lesquerella fendleri protein fractionation and characterization
Author(s) -
Wu Y. Victor,
HojillaEvangelista Mila P.
Publication year - 2005
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-005-1042-0
Subject(s) - glutelin , chemistry , solubility , globulin , sodium hydroxide , sodium , chromatography , nitrogen , fractionation , food science , biochemistry , storage protein , organic chemistry , biology , immunology , gene
Lesquerella fendleri is a promising new crop whose seed contains hydroxy FATG with potential industrial uses as well as substantial amounts of valuable gums. The defatted L. fendleri seeds also contain more than 30% protein. The objective of this study is to process and characterize this protein component for possible future uses in food. Hexane‐defatted seed has more than 30% protein content. Defatted lesquerella meal was extracted sequentially with 0.5 M sodium chloride (2×), water, 70% ethanol, and 0.1 N sodium hydroxide (2×). Each sodium chloride extract was dialyzed against deionized water and centrifuged to separate the water‐soluble fraction (albumin) from the salt‐soluble fraction (globulin) before freeze‐drying. The ethanol extract and the neutralized sodium hydroxide extracts (glutelin) were dialyzed against water and freeze‐dried. Albumin had the highest proportion of lysine and sulfur amino acids per 16 g nitrogen among all the fractions analyzed. Glutelin and globulin accounted for the highest amount of protein nitrogen. SDS‐PAGE of the reduced albumin, globulin, and glutelin showed the presence of several protein bands with M.W. ranging from 7 to 98 kDa. Nitrogen solubility of defatted lesquerella meal from pH 2 to 12 indicated a solubility minimum of 15% around pH 4.2 and a solubility of 75% at pH 11.5. Nonprotein nitrogen of defatted meal was 12% of total nitrogen. Defatted lesquerella meal has the potential for food use based on good nitrogen solubility and good amino acid composition.