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Extracting long‐chain fatty acids from a fermentation medium
Author(s) -
Lalman Jerald A.,
Bagley David M.
Publication year - 2004
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-004-0866-y
Subject(s) - chemistry , capric acid , chloroform , chromatography , caprylic acid , hexane , methanol , extraction (chemistry) , linoleic acid , oleic acid , fermentation , solvent , stearic acid , palmitic acid , ethanol , caproic acid , fatty acid , methyl tert butyl ether , long chain fatty acid , ether , organic chemistry , biochemistry , lauric acid
Several solvents were evaluated for extracting free long‐chain FA (LCFA) from a fermentation medium. Chloroform, chloroform/methanol (1∶1), hexane, and hexane/methyl tert ‐butyl ether (MTBE) (1∶1) were evaluated as alternative extraction solvents. Parameters considered for optimizing LCFA recoveries included pH and ionic strength. Maximal LCFA recoveries were obtained by adding 2 mL of the hexane/MTBE (1∶1) solvent mixture, 80 μL of 50% H 2 SO 4 , and 0.05 g NaCl to 1 mL of the aqueous sample and mixing for 15 min at 200 rpm. This method quantified saturated LCFA [capric acid (C 10∶0 ) to stearic acid (C 18∶0 )] and unsaturated LCFA with 18 carbons [linoleic acid (C 18∶2 ) and oleic acid (C 18∶1 )] with a 98 to 100% recovery. Caproic (C 6∶0 ) and caprylic (C 8∶0 ) acids were characterized by 27 and 76% recoveries, respectively.