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Transesterification of phytosterol and edible oil by lipase powder at high temperature
Author(s) -
Negishi Satoshi,
Hidaka Ichiro,
Takahashi Isamu,
Kunita Shigeru
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0794-x
Subject(s) - phytosterol , transesterification , lipase , sunflower oil , chemistry , food science , organic chemistry , chromatography , enzyme , catalysis
Phytosterol, which is hardly soluble in edible oil, was solubilized at a high concentration by converting it to FA esters by lipase‐catalyzed transesterification at temperatures higher than 100°C using powdered Lipase QLM (Meito Sangyo Co., Ltd., Nagoya, Japan). Transesterification was conducted, in sunflower oil containing 10% phytosterol, without adding water or solvent, at 100°C. The conversion rate was 97.1% after 7 h of reaction. The effect of temperature on the conversion rate was also examined. Maximum enzyme activity occurred in the 100–120°C range, and 20% of the maximum activity was retained even at 130°C. When the lipase was recovered by filtration and recycled for repeated reactions at 90°C, the half‐life of lipase activity was 260 h. Thus, edible oils with nutritional value could be produced by blending the phytosterol‐containing sunflower oil into other edible oils.