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Continuous production of acyl l‐ascorbates using a packed‐bed reactor with immobilized lipase
Author(s) -
Kuwabara Ko,
Watanabe Yoshiyuki,
Adachi Shuji,
Nakanishi Kazuhiro,
Matsuno Ryuichi
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0791-0
Subject(s) - lipase , ascorbic acid , chemistry , candida antarctica , chromatography , critical micelle concentration , aqueous solution , micelle , glycerol , immobilized enzyme , packed bed , surface tension , enzyme , organic chemistry , food science , physics , quantum mechanics
Saturated acyl (6‐ O ‐caproyl, lauroyl, and myristoyl) and unsaturated acyl (6‐ O ‐oleoyl, linoleoyl, and arachidonoyl) l‐ascorbates were continuously synthesized at 50°C using a system where a column packed with ascorbic acid powder and a packed‐bed reactor with an immobilized lipase from Candida antarctica were connected in series. A productivity of 1.6–1.9 kg/L reactor·d was achieved for at least 11 d. The surface tension of the caproyl or lauroyl l‐ascorbate in aqueous solution was measured at various temperatures and pH to estimate the critical micelle concentration (CMC) of the acyl l‐ascorbate. The CMC values were independent of temperature but dependent on the pH. The value of the caproyl ascorbate increased with an increase in pH.