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Lipase‐catalyzed interesterification reaction between menhaden oil and the ethyl ester of CLA: Uniresponse kinetics
Author(s) -
Torres Carlos F.,
Lin Betty,
Lessard Louis P.,
Hill Charles G.
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0788-8
Subject(s) - interesterified fat , chemistry , rhizomucor miehei , menhaden , lipase , catalysis , reaction rate , organic chemistry , kinetics , factorial experiment , chromatography , enzyme , triacylglycerol lipase , fish oil , biology , physics , statistics , mathematics , quantum mechanics , fishery , fish <actinopterygii>
The kinetics of the interesterification reaction between menhaden oil and the ethyl ester of CLA (CLAEE) in the presence of an immobilized lipase from Rhizomucor miehei was investigated. A 2 3 factorial design with a central point was used to define the experimental region. The factors considered were the molar ratio of reactants, the enzyme loading, and the temperature. Optimal results were obtained when the reaction was carried out at 55°C with an enzyme loading of 0.65 g per 12 g mixture and a mole ratio of CLAEE to menhaden oil equal to 0.13. A uniresponse kinetic model of the Michaelis‐Menten type was developed to characterize the rate of consumption of CLAEE and the rate of release of FA ethyl esters from menhaden oil. The best fit of the data with a uniresponse model was obtained using a form based on reversible reactions and inhibition by the ethyl esters of the FA residues released by the reaction.