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Characterization of supercritical fluid extracts from raw wool by TLC‐FID and GC‐MS
Author(s) -
Domínguez C.,
Jover E.,
Garde F.,
Bayona J. M.,
Erra P.
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0763-4
Subject(s) - chemistry , chromatography , supercritical fluid extraction , wax , wool , extraction (chemistry) , solubility , supercritical carbon dioxide , gas chromatography , carbon dioxide , solvent , gas chromatography–mass spectrometry , raw material , lanolin , mass spectrometry , organic chemistry , materials science , composite material
Extraction selectivity of whole wool wax from raw wool by using ethanol‐modified carbon dioxide at constant pressure (100 bar) and temperature (60°C) was studied. A kinetic study was carried out to optimize the percentage of ethanol and the extraction time. The modified carbon dioxide was decompressed in ethanol as a collection solvent, leading to the formation of two different fractions as a function of their solubility. Qualitative and quantitative analyses of the lipid classes present in the collected fractions were performed by TLC coupled to an automated FID system (TLC‐FID). Moreover, a detailed structural comparison was carried out between the aliphatic high‐molecular‐mass esters and the steryl ester patterns by means of subambient pressure GC‐MS in both the EI and the ammonia positive Cl modes. Considerable differences in the lipid composition of the two wool wax fractions collected were observed by TLC‐FID and GC‐MS.