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Reconstitution of single molecular species from isolated subunits of glycinin
Author(s) -
Zhang Guoyan,
Tsunokawa Satoshi,
Hayashi Yukako,
Matsumoto Shinya,
Matsumura Yasuki,
Mori Tomohiko
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0727-8
Subject(s) - protein subunit , chemistry , centrifugation , sucrose , chromatography , molecular mass , differential centrifugation , high performance liquid chromatography , density gradient , amino acid , biochemistry , crystallography , enzyme , gene , physics , quantum mechanics
Ion‐exchange HPLC systems using POROS 20 HQ and Mono Q HR 10/10 columns were applied to isolate glycinin subunits under denaturing conditions. Analyses by SDS‐PAGE, N‐terminal amino acid sequence, and sucrose density gradient centrifugation showed that the pseudoglycinin from the highly purified homo‐subunit, A 3 B 4 , was reconstituted. The A 3 B 4 pseudoglycinin was similar to the native glycinin with respect to molecular size, subunit structure, and secondary structure. The hexameric pseudoglycinin dissociated into trimers after long storage at pH 7.6.