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Selectivity of potato tuber lipid acyl hydrolase toward long‐chain unsaturated FA in esterification reactions with glycerol analogs in organic media
Author(s) -
Pinsirodom Praphan,
Parkin Kirk L.
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0699-8
Subject(s) - chemistry , glycerol , selectivity , alcohol , oleic acid , degree of unsaturation , stearic acid , organic chemistry , oleyl alcohol , propanediol , ethanol , linoleic acid , fatty alcohol , fatty acid , biochemistry , catalysis
Abstract FA selectivity of a Celite‐immobilized potato lipid acyl hydrolase (LAH) in esterification reactions with long‐chain FA, including stearic acid (18∶0), oleic acid (18∶1), linoleic acid (18∶2), α‐linolenic acid (18∶3), EPA (20∶5), and DHA (22∶6), and alcohol co‐substrates ( n ‐propanol, isopropanol, 1,3‐propanediol, and glycerol) was studied in isooctane. Immobilized LAH was selective for FA of greater degrees of unsaturation (18∶3>18∶2>18∶1>18∶0) for all alcohol acceptors evaluated. Selectivity of LAH toward unsaturated C 18 FA increased with an increase in water activity ( a w ) from 0.19 to 0.90 for n ‐propanol, isopropanol, and 1,3‐propanediol as alcohol co‐substrates. In contrast, with glycerol as the alcohol cosubstrate, selectivity of LAH toward these unsaturated C 18 FA increased with a decrease in a w from 0.90 to 0.19. In addition, immobilized LAH strongly discriminated against EPA and DHA for both 1,3‐propanediol and glycerol as alcohol co‐substrates.