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Simultaneous determination of ascorbyl palmitate and nine phenolic antioxidants in vegetable oils and edible fats by HPLC
Author(s) -
Perrin Christian,
Meyer Liliane
Publication year - 2003
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-003-0661-9
Subject(s) - chemistry , citric acid , chromatography , high performance liquid chromatography , methanol , repeatability , ascorbyl palmitate , extraction (chemistry) , food science , ascorbic acid , organic chemistry
This study describes an HPLC method for the simultaneous determination of ascorbyl palmitate (AP) and synthetic phenolic antioxidants (SPA) in vegetable oils and edible fats in a single run. To achieve this, citric acid was used in combination with isoascorbic acid for stabilization of AP in standard and sample solutions and for deactivation of oxidizing agents in the HPLC system. SPA and AP were directly extracted from samples with methanol containing 1 mg/mL each of citric acid and isoascorbic acid. HPLC analytical and guard columns were pretreated with 90% methanol/acetonitrile 1∶1 (vol/vol), containing 4 mg/mL each of citric acid and isoascorbic acid, and 10% water at pH 3, for 30 min. Under these conditions, AP was stable for about 7 h at room temperature. The relative SD of repeatability for AP (0.5–3.6%) was comparable to that for SPA (0.3–2.8%). Average recovery from spiked samples was 100% for AP, 98–103% for SPA, and 85% for BHT (up to 90% using double extraction with methanol).