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Rapid and simultaneous analysis of 16‐ O ‐methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on‐line LC‐GC
Author(s) -
Kamm Willibald,
Dionisi Fabiola,
Fay LaurentBernard,
Hischenhuber Claudia,
Schmarr HansGeorg,
Engel KarlHeinz
Publication year - 2002
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-002-0612-5
Subject(s) - coffea arabica , chromatography , coffea canephora , green coffee , chemistry , food science , biology , botany
An on‐line LC‐GC method for analysis of 16‐ O ‐methylcafestol (16‐OMC) and total sterols in oils obtained from green beans of Coffea canephora (Robusta) and C. arabica (Arabica) has been developed. HPLC pre‐separation of the transesterified lipids allows quantification of the content of 16‐OMC. The fraction containing the sterols is transferred to on‐line GC analysis. The results obtained for Arabica and Robusta samples were in good agreement with literature data obtained by classical analytical procedures. The method allows rapid and simultaneous determination of two markers (16‐OMC and Δ 5 ‐avenasterol) proposed for authenticity assessment of coffee beans and overcomes time‐consuming sample pretreatment steps.