Premium
Enzyme‐assisted aqueous extraction of peanut oil
Author(s) -
Sharma Aparna,
Khare S. K.,
Gupta M. N.
Publication year - 2002
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-002-0463-0
Subject(s) - extraction (chemistry) , peanut oil , aqueous solution , chromatography , chemistry , solvent , hexane , enzyme , proteases , solvent extraction , biochemistry , organic chemistry , raw material
Enzyme‐assisted aqueous extraction of oil from oilseeds is a relatively recent technique. In the present study, peanut oil was extracted under optimized aqueous extraction conditions using Protizyme ™ , which is predominantly a mixture of acid, neutral, and alkaline proteases. The optimal conditions were: enzyme concentration of 2.5% (w/w) in 10 g of peanut seeds, pH 4.0, 40°C, and 18 h incubation with constant shaking at 80 rpm. Centrifuging the mixture at 18,000 × g for 20 min separated the oil with a recovery of 86–92%. The merits of this process over existing solvent extraction and/or mechanical pressing methods are discussed.