Premium
Conjugated linoleic acid production from linoleic acid by lactic acid bacteria
Author(s) -
Kishino Shigenobu,
Ogawa Jun,
Omura Yoriko,
Matsumura Kenji,
Shimizu Sakayu
Publication year - 2002
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-002-0451-4
Subject(s) - conjugated linoleic acid , linoleic acid , chemistry , lactic acid , food science , lactobacillus plantarum , bacteria , substrate (aquarium) , yield (engineering) , biochemistry , fatty acid , biology , materials science , metallurgy , genetics , ecology
After screening 14 genera of lactic acid bacteria, Lactobacillus plantarum AKU 1009a was selected as a potential strain for CLA production from linoleic acid. Washed cells of L. plantarum with high levels of CLA production were obtained by cultivation in a nutrient medium with 0.06% (wt/vol) linoleic acid ( cis ‐9, cis ‐12‐octadecadienoic acid). Under the optimal reaction conditions with the free form of linoleic acid as the substrate, washed cells of L. plantarum produced 40 mg CLA/mL reaction mixture (33% molar yield) from 12% (wt/vol) linoleic acid in 108 h. The resulting CLA was a mixture of two CLA isomers, cis ‐9, trans ‐11 (or trans ‐9, cis ‐11)‐octadecadienoic acid (CLA1, 38% of total CLA) and trans ‐9, trans ‐11‐octadecadienoic acid (CLA2, 62% of total CLA), and accounted for 50% of the total FA obtained. A higher yield (80% molar yield to linoleic acid) was attained with 2.6% (wt/vol) linoleic acid as the substrate in 96 h, resulting in CLA production of 20 mg/mL reaction mixture [consisting of CLA1 (2%) and CLA2 (98%)] and accounting for 80% of total FA obtained. Most of the CLA produced was associated with the cells ( ca . 380 mg CLA/g dry cells), mainly as FFA.