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A simple HPLC method for the simultaneous analysis of phosphatidylcholine and its partial hydrolysis products 1‐ and 2‐acyl lysophosphatidylcholine
Author(s) -
Adlercreutz Dietlind,
Wehtje Ernst
Publication year - 2001
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-001-0379-8
Subject(s) - lysophosphatidylcholine , chemistry , chromatography , elution , oxalic acid , high performance liquid chromatography , hydrolysis , ethanol , phosphatidylcholine , organic chemistry , biochemistry , phospholipid , membrane
Abstract A simple HPLC method for the simultaneous analysis of phosphatidylcholine (PC), 1‐acyl lysophosphatidylcholine (1‐acyl LPC), and 2‐acyl lysophosphatidylcholine (2‐acyl LPC) with refractive index detection is described. The separation of these three compounds was achieved on a Waters (Milford, MA) Spherisorb amino phase column using a mixture of ethanol and an aqueous oxalic acid solution as eluent. The optimal mixture of ethanol to oxalic acid solution was 92∶8 (vol/vol). PC and the two regioisomers of LPC eluted within 15 min. The calibration curves were linear in a concentration range from 0.05 to 2.5 mM. Natural PC or LPC eluted in a single peak, despite the diversity in the fatty acid composition. There was no rearrangement between 1‐acyl LPC and 2‐acyl LPC during analysis or storage in ethanol within 23 h. This method is thus especially suitable for studying reactions on PC and acyl migration in LPC.