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Identification of ( e )‐11‐hydroxy‐9‐octadecenoic acid and ( E )‐9‐hydroxy‐10‐octadecenoic acid by biotransformation of oleic acid by Pseudomonas sp. 32T3
Author(s) -
Rodríguez E.,
Espuny M. J.,
Manresa A.,
Guerrero A.
Publication year - 2001
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-001-0310-3
Subject(s) - oleic acid , bioconversion , chemistry , biotransformation , chromatography , mass spectrometry , substrate (aquarium) , organic chemistry , gas chromatography–mass spectrometry , biochemistry , enzyme , biology , fermentation , ecology
Pseudomonas sp. 32T3, a newly identified strain originally isolated from a vegetable oil‐contaminated soil, produces three monohydroxy acids—( E )‐11‐hydroxy‐9‐octadecenoic acid, ( E )‐10‐hydroxy‐8‐octadecenoic acid, and ( E )‐9‐hydroxy‐10‐octadecenoic acid—as bioconversion products of oleic acid. The bacterial cells were grown in a mineral medium containing oleic acid as the main carbon substrate. The compounds were identified as the corresponding methyl esters on the basis of their chromatographic and spectroscopic ( 1 H and 13 C nuclear magnetic resonance and gas chromatography‐mass spectrometry) features.