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Lipase‐catalyzed hydrolysis of canola oil in supercritical carbon dioxide
Author(s) -
Rezaei Karamatollah,
Temelli Feral
Publication year - 2000
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-000-0143-0
Subject(s) - chemistry , supercritical carbon dioxide , hydrolysis , supercritical fluid , canola , chromatography , lipase , catalysis , monoglyceride , organic chemistry , carbon dioxide , chemical engineering , food science , enzyme , fatty acid , engineering
The effect of pressure, temperature, and CO 2 flow rale on the extent of conversion and the product composition in the enzyme‐catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO 2 ) was investigated using lipase from Mucor miehei immobilized on macroporous anionic resin (Lipozyme IM). Reactions were carried out in a continuous flow reactor at 10, 24, and 38 MPa and 35 and 55°C. Supercritical fluid chromatography was used to analyze the reaction products. A conversion of 63–67% (triglyceride disappearance) was obtained at 24–38 MPa. Mono‐and diglyceride production was minimum at 10 MPa and 35°C. Monoglyceride production was favored at 24 MPa. The amount of product obtained was higher at 24–38 MPa due to enhanced solubility in SCCO 2 . Complete hydrolysis of oil should be possible by increasing the enzyme load and/or decreasing the quantity of the oil substrate. There was a drop in triglyceride conversion over a 24‐h reaction time at 38 MPa and 55°C, which may be an indication of loss of enzyme activity. Pressure, temperature, and CO 2 flow rate are important parameters to be optimized in the enzyme‐catalyzed hydrolysis of canola oil in SCCO 2 to maximize its conversion to high‐value products.