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Enrichment of eicosapentaenoic acid and docosahexaenoic acid in saponified menhaden oil
Author(s) -
Chen TorChern,
Ju YiHsu
Publication year - 2000
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-000-0068-7
Subject(s) - saponification , docosahexaenoic acid , chemistry , eicosapentaenoic acid , acetone , solvent , yield (engineering) , solubility , chromatography , menhaden , fish oil , organic chemistry , nuclear chemistry , fatty acid , polyunsaturated fatty acid , materials science , fishery , fish <actinopterygii> , metallurgy , biology
Eicosapentaenoic acid (EPA, 20∶5n−3) and docosahexaenoic acid (DHA, 22∶6n−3) in free fatty acids (FFA) derived from saponified menhaden oil were concentrated by the solubility differences of FFA‐salts in organic solvent. FFA‐salts were formed by adding NaOH to a solution containing FFA. A Buchner funnel was used to separate solid phases from liquids containing FFA‐salts. FFA that are rich in EPA and DHA can be recovered from the liquid phase by the addition of 12 N HCl. The effects of reaction time, the amount of NaOH, and solvent used on the concentration of EPA and DHA were systematically investigated. With a total volume of 112 mL, made up of 1.85% 15 N NaOH, 88.1% acetone, and 10.0% FFA, a reaction temperature of 30°C, and a reaction time of 1 h, the resulting liquid phase contained 65.4 wt% EPA and DHA, with a corresponding yield of 41.5%. By replacing the acetone with a mixture of 45% acetone and 55% acetonitrile and then storing the liquid phase at −70°C overnight, the content and yield of EPA and DHA in the final liquid phase were 61.4 wt% and 66.2%, respectively.